Abstract—In view of the low content of lipase in rice bran, it
is difficult to directly and efficiently measure its lipase activity
in the rice bran heat inactivation experiment, an experimental
method of adding exogenous lipase was proposed to quickly and
accurately detect the lipase activity in the heated rice bran.
Taking the correlation between the temperature and time of rice
bran heat treatment and the enzyme activity as the starting
point, the self-made aluminum heating container with high
sealing property was used, and the COMSOL simulation
verified that the container met the uniformity requirements of
rice bran heating; Take rice bran as the heating medium, add a
certain amount of exogenous plant lipase, and then heat the rice
bran containing exogenous lipase, and detect the activity of
lipase after heating, to explore the application of adding
exogenous lipase in rice bran stabilization. The results showed
that when exogenous lipase and rice bran were mixed with 1:1
(mass ratio), the lipase activity of the mixture increased in equal
proportion; After heat treatment at 110 ℃, 120 ℃ and 130 ℃,
the measured change of lipase activity in the mixture was in good
agreement with the change trend of rice bran lipase activity, and
the difference between different test temperature groups was
significant (P<0.05), which provided an ideal detection model for
the detection of lipase activity in the study of rice bran
stabilization.
Index Terms—Rice bran, lipase activity, stability, exogenous
lipase
The authors are with the Mechanical and Electronic engineering
Department, Northwest Agriculture & Forestry University, 712100 Shaanxi,
P. R. China.
*Correspondence: gufang@nwafu.edu.cn (W.F.G)
[PDF]
Cite: Mingyue Deng, Haixu Liu, Yongze Chai, Shaojin Wang, and Fang Gu, "Application of Exogenous Lipase in the Study of Rice Bran Stabilization," International Journal of Engineering and Technology vol. 15, no. 4, pp. 166-171, 2023.
