Abstract—The identification of biological aerosols is a
subject of worldwide interest because of the potential for
biological terrorism and the increase in public health
emergencies. Laser-induced fluorescence is widely used to
investigate the fluorescence properties of biomolecules and
spores in solution; however, data on the fluorescence properties
of the aerosolized forms are lacking. In this study, we report the
fluorescence properties of tryptophan, serotonin, tyrosine,
vitamin B6, and Bacillus thuringiensis and Bacillus subtilis
spores in solution and aerosol form. Our results show that
the shapes of the fluorescence spectra were generally consistent
between the solution and aerosol states despite some wavelength
shifts in the emission spectra. In contrast, the fluorescence
intensity of the emissions varied significantly between the
solutions and aerosols. Given the growing global interest in
accurate measurement of airborne biological molecules and
particles, our findings provide a useful baseline for further
research into the development of laser-induced
fluorescence-based bioaerosol detection instruments and can
guide the ongoing design of effective measurement and
engineering technologies.
Index Terms—Emerging technologies, hardware,
laser-induced fluorescence method, optical and photonic
technologies
Yanlei Li, Yi Cai, Zhe Yang, Haiyan Chen, Zhanying Zhang, Dongsheng
Yu, Weikai Fang, and Chidong Xu are with Anhui Institute of Optics and
Fine Mechanics, Chinese Academy of Sciences, Hefei, Anhui, China.
Yanfei Wang is with the Hefei Normal University, School of Physics and
Materials Engineering, Hefei, Anhui, China.
*Correspondence: xcd@aiofm.ac.cng (C.X.)
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Cite: Yanlei Li, Yi Cai, Zhe Yang, Haiyan Chen, Zhanying Zhang, Dongsheng Yu, Weikai Fang, Yanfei Wang, and Chidong Xu, "Comparison of the Fluorescence Properties of Biological Solutions and Aerosols," International Journal of Engineering and Technology vol. 15, no. 3, pp. 115-118, 2023.